Obtaining molecules with optimal performance

DEINOVE’s Genetic engineering platform works towards genetically optimising bacterial strains for the production of a given compound. This includes metabolically modifying the bacteria to produce large amounts of the compound, therefore ensuring the best downstream production efficiency, as well as ensuring that the bacteria can withstand industrial scale fermentation conditions.

Working with rare bacterial strains

One limitation of working with rare bacterial strains is the lack of knowledge as to how these organisms can be genetically modified. DEINOVE has developed a large experience in handling and modifying bacteria of the Deinococcus genus, and has built on this experience to now handle diverse bacterial strains. New organisms are taken through a pipeline of standardized genetic modification techniques to define within 4 months a toolbox of methods that work for each one of them.

A computer-aided, high-throughput genetic engineering platform

The metabolic pathways leading to the production of most compounds are often complex and involve many different steps and enzymes along the way. Optimising a metabolic pathway to produce a given compound therefore requires modulating the expression levels of several genes simultaneously. Thousands of strains with differences in the expression of these genes often need to be constructed before landing the one strain with the optimal production of the compound of interest.

DEINOVE has worked in collaboration with CAD4Bio to develop a custom made computer- aided design software that designs complex combinatorial DNA libraries, generates assembly instructions and operates a robot for high-throughput construction. Today, DEINOVE is able to produce 1000 different bacterial strains in a month. To optimize strain construction even further, the CAD4Bio software was recently improved with a machine-learning component. Using the outcomes of previous experiments, it can now predict the feasibility and probability of success  of future constructs, therefore reducing the time needed to generate strains with the optimal metabolic equilibrium.

 

Key features

 

 

Large experience handling and developing techniques for Deinococcus: adaptability to new rare organisms

Computer-aided genetic design with machine-learning component using a custom made CAD4Bio software

 

 

High-throughput, automated strain construction

Can make up to 1,000 strains per month